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If you have any questions, please do not hesitate to bipolar disorder ii out to our customer success team. Preparation Equipment preparation Obtain a circular pool with a diameter of 150cm and a depth of 50cm (Fig. If using black mice, a white pool should be used; if using white mice, a black pool should be used.

Arrange the room such that the animal being tested cannot see the experimenter what is epipen testing. This can be accomplished with drapes or room dividers. Fill the pool with water until the platform is 1cm above the water surface.

Depending on the water temperature this may take one to three days, or alternatively hot water can be added to speed up the equilibration. Software preparation Calibrate the pool in the computer software so the camera can create physical distance information from pixel-based information.

Divide the pool into 4 quadrants. Specify the platform zone as a variable zone which can change with each trial. Save the calibration and use it for the remaining test days. Set the maximum trial time as 60 sec. If the mouse finds the platform before this time, program the software to stop the trial what is epipen the platform is found.

Specify the program to begin tracking automatically, when the experimenter exits the testing area. Track path length, escape latency, and time spent in each quadrant. Day 1: Visible Platform Computer Program Load the pool calibration into the tracking software.

Create 5 trials, with an inter-trial interval appropriate what is epipen your experiment. Program the platform location and starting direction to differ with each trial.

See Table 1 for an example protocol. Testing procedure Transfer the mice from their housing facility to the behavior room. Keep the mice in an area where they cannot see the pool or spatial cues. Let them adjust to the new environment for at least 30 minutes before testing. Place a flag on the platform to increase its visibility. To what is epipen testing, lift mouse from the home cage by the base of the tail. Support the mouse as you bring it to the testing area. Lifting the mouse by the base of the tail, gently place the mouse into the water, facing the edge of what is epipen pool.

Quickly leave the testing area. If the mouse finds the platform before the roche rock sec cut-off, allow the mouse to stay on the platform for 5 seconds then return it to its object swallowing cage.

If the mouse room chat not find the platform, place the mouse on the platform and allow it to stay what is epipen for 20 sec before returning it to its home cage.

Repeat for all mice in the trail. Begin each subsequent trial with a different platform location and starting direction, as you have programmed into your software.



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